Yes, you can create as many subgroups and sub-subgroups as you need, which is perfect for better management!
This is especially helpful if you have students of different ages and want to create subgroups based on their age groups.
How to create a subgroup
From your Enterprise account, click on Manage in the top menu and select Groups:
Select the group you want to add a subgroup to
Under the Subgroups tab click Create:
Add your subgroup’s name and the number of Gold seats you want to assign to this subgroup, then click Create
You'll see a banner when the subgroup has been created
You can also create Subgroups inside Subgroups:
Check out this article to learn how to delete a subgroup: Can I delete organizations/groups/subgroups?